Figure 7
IPA-predicted activation of cardiac fibrosis and glucose metabolism disorder networks, immune dysfunction, and visualization of cardiac fibrosis in ZDF-M. IPA predicted networks show that (a) cardiac fibrosis and glucose metabolism disorder are activated, while (b) “cell viability of lymphocytes”, “quantity of regulatory T lymphocytes”, “stimulation of mononuclear leukocytes”, and “differentiation of mononuclear leukocytes” are inhibited. In support of cardiac fibrosis, representative images of (c) Trichrome stained heart sections of ZL-F, ZDF-F, ZL-M, and ZDF-M are shown. Images were taken at 10x magnification (scale bars = 75 µm). (d) Graph shows the normalized fibrotic area from 10 images for each animal (n = 6 animals per group). (e) Immunohistological staining for COL1A in heart sections of ZL-F, ZDF-F, ZL-M, and ZDF-M are shown. Images were taken at 63X magnification (scale bars = 75 µm). (f) Graph shows the fluorescence intensity quantified from images (n = 3–6 animals per group).
