Figure 3
(A) Aag2 cell line transfected with antagomir for miR-2b, miR-989, and miR-100, 24 and 48 h after transfection, showing expression levels of ubiquitin, URM, sh2/sh3 adaptor, vacuolar ATP synthase, cdc42, and sumoligase. (B) Mosquito injected with antagomir for miR-2b, miR-989, and miR-100, 24 and 48 h after transfection, showing expression levels of ubiquitin, URM, sh2/sh3 adaptor, vacuolar ATP synthase, cdc42, and sumoligase. (C) Luciferase assay showing relative percentage of luciferase/renilla luminescence for miR-2b binding to 3′UTR of URM and to mutated 3′UTR of URM. Data were expressed as mean ± SEM except in 3 C, which is expressed in SD; ****p < 0.0001 vs. control group.
