Figure 4

Genomic distribution of major repeats in B. oleracea through FISH analysis. (A) FISH analysis of major Brassica repeats. Green and pink arrows indicate major STR-Br and STR-Bo signals, respectively, amounting to three pairs of BoSTR signals (as observed by Koo et al., 2011.) (B) Karyogram of B. oleracea based on the distribution of major DNA tandem repeats. Blue-green arrows indicate chromosomes with very weak CentBo1 but strong CentBo2 signals. CentBo1 and CentBo2 are colocalized on most other chromosomes. Green and pink arrows indicate major signals of STR-Br and STR-Bo, respectively, which amounted to three major signals. Bar = 10 µm. (C) Karyotype idiogram of B. oleracea. Centromeric bars represent arbitrary signal hybridization intensities.