Figure 3
From: Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway
The activities of NF-κB and secretion of IL-8 were significantly repressed in H19 silenced MM cells. Secretions of IL-6 by hBMSCs were significantly decreased when co-cultured with H19 silenced MM cells. (A) Phosphorylated IκBα and P65, as well as cytosol and nucleous protein levels of P65 in H19 silenced MM cells were detected by western blot. GAPDH expression was used to normalize for equal loading. TNF-α (100 mg/mL for 24 h) was used to induce NF-κB activation. Secretions of IL-8 by MM cells (B) and IL-6 by hBMSCs co-cultured with MM cells (C) were detected by ELISA. (*P < 0.01, Figure is representative of 3 experiments with similar results).
