Figure 8
From: Fibronectin is a stress responsive gene regulated by HSF1 in response to geldanamycin
A subset of other extracellular matrix genes are stress responsive. (A) The −2000 bp to + 1 bp promoter regions of selected genes were analysed using TFSearch. Fibronectin 1 (FN1), laminin β3 (LAMB3), laminin γ2 (LAMC2), collagen 4 α2(COL4A2), collagen 4 α3 (COL4A3), elastin (ELN), vitronectin (VTN), osteopontin (SPP1), heat-shock factor 1 (HSF1), hypoxia inducible factor α (HIF1α), Hsp90α (HSP90AA1), Hsp90β (HSP90AB1), Hsp72 (HSPA1A). The locations of predicted heat-shock elements (HSE) are indicated by black boxes. Hs578T cells were treated with GA 100 nM and the relative mRNA levels from known stress inducible genes (B) and a selection of ECM genes (C) was assessed by qRT-PCR. Data shown are average mRNA abundance (ΔCt) for genes in the GA treated sample relative to the DMSO treated control (which is 1 in all cases), calculated from 3 independent biological experiments each run in triplicate. Statistical analysis was conducted in comparison to the reference control (ACTB) using one way ANOVA with Bonferroni post-test (*p < 0.05, ***p < 0.001).
