Figure 5

Lack of NET, LNG and progesterone on TFV and TAF inhibition of HIV infection of and intracellular TFV-DP concentration in blood CD4+ T cells. Activated blood CD4+ T cells were pre-treated with TFV at 5 µM (a) or TAF at 5 nM (c) for 24 h and inoculated with HIV for 2hr. Levels of p24 released into the culture media presence or absence of NET, LNG or progesterone (P) at 1 × 10⁻⁷ M for 5 days following infection were measured by p24 enzyme-linked immunosorbent assay. Data were normalized to % of HIV infection with respect to infected control without TFV or TAF pre-treated and in the absence of MPA (BaL only). TFV-DP concentrations were measured by LC-MS/MS in purified blood CD4+ T cells pre-treated with NET, LNG and progesterone at 1 × 10⁻⁷ M for 24hr following treated with (b) TFV (3277 µM) and (d) TAF (2 µM) for 24 hr. Data were normalized to % fold change from control values. (Each horizontal line represents the mean and SEM from 3 individual blood donors.