Figure 2
From: Econazole nitrate inhibits PI3K activity and promotes apoptosis in lung cancer cells
Econazole specifically inhibits the PI3K/AKT pathway in lung cancer cell lines. (A) The putative PI3K/AKT signaling pathway. (B) H661 and A549 cells were treated with increasing concentrations of econazole (1.25, 2.5, 5, or 10 μM) for 24 hours. The expression levels of p-AKT (S473 and T308) and Bcl-2 were decreased by econazole treatment in a dose-dependent manner. The expression of PI3K p110α in H661 but not A549 cells was decreased by econazole treatment, and econazole had no effect on PI3K p110δ in both cells. The expression levels of t-AKT, ERK, p-ERK, VEGF and MMP-9 were not changed by econazole treatment. Normalized band intensities were added below each band.
