Figure 5
From: Shape analysis of the basioccipital bone in Pax7-deficient mice
Pax7 expression in the murine basioccipital bone. Representative bright field (1) and fluorescent images (tdTomato, red (2); GFP, green (3)) of the dorsal aspect of basal occipital bones were obtained from 4 to 5-week-old (A) Pax7 ICNm/WT Rosa WT/WT (Pax7ICN), (B) Pax7 WT/WT Rosa nT-nG/WT (RosanTom/nGFP), and (C) Pax7 ICNm/WT Rosa nT-nG/WT (Pax7ICN RosanTom/nGFP) mice. Rostral region is visualized on the right and the caudal region on the left of each image. Eight mice were analyzed per genotype. White dots denote the anatomical boundary of the basioccipital bone. Eight mice were analyzed per genotype. Three of the eight Pax7 ICNm/WT Rosa nT-nG/WT mice did not demonstrate RFP or GFP fluorescence, which was attributed to transgene silencing for the reporter allele. Images were acquired at 7X magnification.
