Figure 1

AR overexpression sensitizes AURKA under androgen regulation. LNCaP-cells were stably transfected to overexpress 8- to 10-fold higher wt-AR (LNCaP-ARhi) compared to that in empty vector LNCaP-pcDNA3.1 cells. Bar plots show the relative expression quantified with qRT-PCR and normalized to TBP. Relative KLK3 expression in (a) LNCaP-pcDNA3.1 and (b) LNCaP-ARhi cells and relative AURKA expression in (c) LNCaP-pcDNA3.1 and (d) LNCaP-ARhi after 8 and 12 h of treatment with DHT (10 ng) with and without CHX (10 µg). *P < 0.05 and **P < 0.01, unpaired t test.