Figure 1

Generation of global Ces1/Ces1g ^−/− mice and Ces1/Ces1g tissue distribution. (A) A diagram showing the Ces1/Ces1g wild-type (WT) allele and the targeting allele. Exons 1 and 2 are replaced by a nucleus LacZ (nLacZ) cassette and neomycin cassette. K, KpnI. B, BamHI. (B) Genomic DNA from WT or heterozygous ES cells was used for Southern blotting using a 5′ probe (left panel) or 3′ probe (right panel). (C) Mouse genomic DNA was genotyped by PCR. (D) Hepatic mRNA levels of different Ces1 isoforms were determined (n = 4). (E) LacZ staining of various tissues isolated from Ces1/Ces1g ^+/− mice. (F) Hepatic proteins were isolated from 8-weeks-old male control littermates (Ces1/Ces1g ^+/+ mice) or Ces1/Ces1g ^−/− mice. Protein levels were determined by Western blotting. The top band is Ces1/Ces1g. (G) LacZ staining of peritoneal macrophages isolated from Ces1/Ces1g ^+/− mice. (H) Protein levels in the liver (serve as control) or peritoneal macrophages of Ces1g ^+/+ or Ces1g ^−/− mice were determined by Western blotting. BAT, brown adipose tissue. WAT, white adipose tissue. GB, gallbladder. The Western blots are cropped from larger gels.