Figure 2

Ces1/Ces1g ^−/− mice have reduced plasma cholesterol levels. (A and B) Male Ces1/Ces1g ^−/− mice and their control littermates (Ces1/Ces1g ^+/+) were fed a chow diet for 6 months (n = 8). Plasma TG (A) and cholesterol (B) were determined. (C-G) 8-weeks old male Ces1/Ces1g ^−/− mice and their control littermates (Ces1/Ces1g ^+/+) were fed a Western diet for 16 weeks (n = 8). Plasma TG (C) and cholesterol (D) levels were determined. FPLC was performed to determine cholesterol distribution in lipoproteins (E). Representative liver images of oil red O staining are presented (F). Hepatic mRNA levels of inflammatory marker were analyzed (G). *P < 0.05, **P < 0.01.