Table 1 Opines and amino acids affinity measurement for OccJ, NocT, LAO and mutants. K D values were measured by intrinsic protein fluorescence titration (F) and/or by isothermal titration microcalorimetry (ITC). NI means no interaction whereas a white case means experiment not done.
Protein | Octopine K D μM | Nopaline K D μM | L-arginine K D μM | L-lysine K D μM | ornithine K D μM | |
|---|---|---|---|---|---|---|
OccJ WT | F/ITC | 0.004 ± 0.0001/0.009 | NI | 7.2 ± 1/35.7 ± 2.1 | 7.5 ± 2.1/71 ± 6 | 31.2 ± 3.5/40.5 ± 15 |
OccJ S91G | F/ITC | 29.9 ± 3.8/20 ± 3 | NI | NI | ||
OccJ N202D | F/ITC | 0.006 ± 0.001/0.016 ± 0.008 | NI | 7.5 ± 1.2/20.4 ± 4 | ||
NocT WT | F/ITC | 6.1 ± 0.7/9.9 ± 0.7 | 3.7 ± 0.6/ | NI | NI | NI |
NocT G97S | F/ITC | 63.8 ± 13.8/52 ± 10 | NI | NI | ||
LAO WT | ITC | NI | NI | 0.024 ± 0.002 | ||
LAO Q122A | ITC | 34 ± 5 | NI | 0.12 ± 0.01 |
