Figure 1 | Scientific Reports

Figure 1

From: CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex

Figure 1

Immunological detection of CpRLP1 protein in C. psl. complex cells. (a) CpRLP1 expression during sexual reproduction. Cells of the mt+ and mt− strains were co-incubated in nitrogen-depleted medium and CpRLP1 levels were monitored by immunoblot analysis at various time points after co-incubation. (b) Cells of the mt− strain were incubated in nitrogen-depleted medium containing PR-IP. Protein was isolated at regular time intervals and subjected to SDS-PAGE followed by immunoblotting with anti-CpRLP1 antibodies.

Back to article page