Figure 3 | Scientific Reports

Figure 3

From: CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex

Figure 3

Overview of CRISPR/Cas9-induced mutations in CpRLP1. The wild-type (WT) sequence is shown with the protospacer adjacent motif (PAM) sequence in orange and the sgRNA target sequences (target region A and B) in blue. The corresponding amino acid (AA) sequence is depicted above the nucleotide sequence. Deletions are denoted using red dashes. Insertions and nucleotide substitutions are indicated in red letters. The amino acid sequences of the target region are listed on the right, and the altered sequences are indicated using red letters and dashes.

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