Figure 5 | Scientific Reports

Figure 5

From: CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex

Figure 5

Effect of PR-IP on the induction of protoplast release. (a) Photographs of control and CRISPR/Cas9-mediated CpRLP1 knockout mt− cells (CpRLP1-KO) incubated with various concentrations of PR-IP. The amount of PR-IP per 2 ml of MI medium is shown above, and the names of strains are indicated to the left. Arrowheads indicate protoplast-releasing cells. Scale bar = 50 µm. (b) Proportion of cells releasing protoplasts in the presence of various concentrations of PR-IP. The dashed and solid lines represent data for the CpRLP1 knockout strains (A-2, B-2, and B-4) and control strain (C-2), respectively. The data represent averages of three independent experiments. Vertical bars indicate SE.

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