Figure 2 | Scientific Reports

Figure 2

From: Differential proteomics of lesional vs. non-lesional biopsies revealed non-immune mechanisms of alopecia areata

Figure 2

MS/MS identification and quantitative analysis of vimentin. (A) Isotope view of the selected MS precursor that was subjected to MS/MS peptide sequencing. (B) Graphical illustration of individual amino acid residues derived from MS/MS spectra that significantly matched to a peptide “ILLAELEQLK” of vimentin. (C) MS/MS ion table displaying the calculated mass of possible fragment ions used for such amino acid matching. In (B,C), fragment ions that were derived from N- and C-terminal scans of the spectra are highlighted in red and blue, respectively. (D) Representative MS/MS spectra of vimentin that were assigned for quantitative analysis to compare lesional vs. non-lesional biopsies.

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