Table 2 Primers and product size of genes used for validation using quantitative real-time polymerase chain reaction.

Gene symbol^#	GenBank accession number	Forward (F) primers (5′-3′)	Product size (base pair)
Gene symbol^#	GenBank accession number	Reverse (R) primers (5′-3′)	Product size (base pair)
Hsp25	NM_001010842	F: CCGTCTTCTGCTGAGAGGAGTG	117
		R: ACCGTTGTTCCGTCCCATCAC
Hsp90aa1	NM_001109785	F: GGTGTTGGTTCCTACTCTGCTTAC	76
		R: ACTGCTCATCATCATTGTGCTTGG
Cirbp	NM_001031347	F: GCCTGGGTACAAATTGGAAG	72
		R: GCAGGTTGAACATACAAGCAAG
ApoB	NM_001044633	F: TTCCAGCTTCCACGTATCCC	181
		R: ATTTGGACGTTGCTTGAGCTG
Prdx4	BX935739	F: ACATGCACTTAGGGGCCTTTT	90
		R: TCCACTGATCTCCCCACAGG
Cyp19a1	NM_001001761	F: AGACGGTGCAGAGTACAGAC	92
		R: AGGCTGCCTTTCTATTGGGTG
Hsd17b1	NM_204837	F: CTCGGAGCAGGCCATGAGAG	70
		R: GAAGGCCTGAATGGTGCGT
Serpinh1	NM_205291	F: AGCGCCCTGAAATCCATCAA	171
		R: GAAGCCACGGTTATCCACCA
18s rRNA	KT445934.2	F: GGGATGCAGATCTTCGTGAAA	117
		R: CAAAAGCTTGTGTCGAGGGC

^#Abbreviations: Hsp25, heat shock protein 25 gene; Hsp90aa1, heat shock protein 90 alpha gene; Cirbp, cold-inducible RNA-binding protein gene; ApoB, apolipoprotein B gene; Prdx4, peroxiredoxin 1 gene; Cyp19a1, cytochrome P450, family 19, subfamily A, polypeptide 1 gene; Hsd17b1, hydroxysteroid 17-beta dehydrogenase 1 gene; Serpinh1, heat shock protein 47 gene.

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