Figure 2

Extracellular superoxide anion (O2 −) production in HKL subjected to the experimental treatments. The AA treatments depicted in the X-axis are as follows: L-15, control; G1, 4.1 mM L-glutamine; G2, 5.1 mM L-glutamine; A1, 5.7 mM L-arginine; A2, 7.2 mM L-arginine; T1, 0.2 mM L-tryptophan; T2, 0.25 mM L-tryptophan; M1, 1 mM L-methionine; M2, 1.25 mM L-methionine. Values represent means ± SD (n = 6 biological replicates). Different low case letters denote statistically significant differences between AA treatments, regardless stimulation or incubation time (Multifactorial ANOVA; Tukey post-hoc test; P ≤ 0.05).