Figure 3
Extracellular ATP concentration in the supernatant of HKL subjected to the experimental treatments for 4 (a) or 24 h (b) under CTRL (
) or vaLPS (
) conditions. The AA treatments depicted in the X-axis are as follows: L-15, control; G1, 4.1 mM L-glutamine; G2, 5.1 mM L-glutamine; A1, 5.7 mM L-arginine; A2, 7.2 mM L-arginine; T1, 0.2 mM L-tryptophan; T2, 0.25 mM L-tryptophan; M1, 1 mM L-methionine; M2, 1.25 mM L-methionine. Values represent means ± SD (n = 6 biological replicates). * and # stand for statistically significant differences between stimuli within the same AA treatment and time of incubation. Different low case letters denote statistically significant differences between AA treatments within the same stimuli and incubation time. x and y stand for statistically significant differences between incubation time, within the same AA treatment and stimuli (Multifactorial ANOVA; Tukey post-hoc test; P ≤ 0.05).
