Figure 3
From: New Types of Experiments Reveal that a Neuron Functions as Multiple Independent Threshold Units
Variability in the Spike Waveform as a function of the Stimulation Location. (A) The alternating stimulation scheduling for this type of experiments. The patch neuron is alternatingly stimulated by two extracellular electrodes (green and pink, see also Fig. 2A2 ) at a low frequency, 0.5 Hz. Each colored rectangle represents a stimulation by the corresponding extracellular electrode (the width is arbitrary, see Methods for details), similar to the realization illustrated in Fig. 2A2 . (B) An example of intracellular recording from a patch neuron stimulated alternatingly, as in (A), showing two different well-separated spike waveforms. The voltage is presented from 5 milliseconds prior to the threshold crossing, which is defined at -50 mV. (C) An illustration of a neuron stimulated above-threshold either via the green dendrite (C 1 ) or via the pink dendrite (C 2 ), where each one generates a different waveform for the spike (colored coded). The suitable neuronal model for the presented experimental results is model III in Fig. 1C, where when the green weight crosses its spring threshold a “green” spike is evoked, while when the pink weight crosses its spring threshold a “pink” spike is evoked. (D) Examples of different spike waveforms recorded intracellularly and generated by two extracellular stimulating electrodes (pink and green) with reliable evoked spikes (Fig. 2C). Each one of the eight panels is associated with a different neuron, and for each two electrodes two evoked spikes are plotted to illustrate the reproducibility of the spike waveform.
