Figure 2
From: Nanopore DNA Sequencing and Genome Assembly on the International Space Station
Automated metagenomic analysis of ISS nanopore data. (A) Flow chart of the SURPIrti bioinformatics pipeline for real-time microbial detection from nanopore data. (B) Donut charts of read distributions corresponding to all reads (left), bacteria (middle), and viruses (right) from pooled ISS runs 1 through 8. (C) Stacked column plot of reads each from ISS runs 1 through 8 showing distribution of identified organisms. (D) Stacked: bar plot of reads from pooled ISS runs 1 through 8 comparing metagenomic detection using SURPIrt versus directed alignment using GraphMap for organism identification from nanopore sequencing data.). (E) Coverage (green) and pairwise identity plots (purple) of raw nanopore reads mapped to the E. coli (upper panel), the mouse mitochondrial (lower left panel), and lambda genomes (lower left panel). Reads are mapped to the most closely matched reference genome identified by SURPIrt. Images not generated by the authors were obtained from the CDC Public Health Images Library: human silhouette, image ID 15798, illustrator D. Higgins; giardia, image ID 3394, source A. da Silva and M. Moser; yeast, image ID 300, no attribution possible; virus, image ID 21351, illustrator, A. Eckert; bacteria, image ID 21915, A. Eckert and J. Oosthuizen.
