Figure 5
FGF21 ameliorated insulin resistance in HK-2 cells. HK-2 cells were pretreated with PA (0.5 mM) for 48 h, and stimulated by 100 nM insulin in the presence of FGF21 (0, 50 ng/ml, 100 ng/ml, 200 ng/ml). Summarized data showing 2-NBDG uptake (a) and MFI for expression of GLUT4 in plasma membrane by flowcytometry (b). Representative Western blot gel documents and summarized data showing the protein expression of pIRS-1ser307 (c) and p-AKT (d) in HK-2 cells. *P < 0.05 vs. Ctrl; # P < 0.05 vs. PA treated group (n = 3).
