Figure 6
PGE1 alleviated HK-2 cell insulin resistance through FGF21 signaling pathway. HK-2 cells were incubated with PA (0.5 mM) for 48 h, which was treated with PGE1 (0.1 ng/ml) and insulin (100 nmol/L) in the presence or absence of the transfection of ATG7 siRNA or PD173074. Summarized data showing -NBDG uptake (a) and MFI for expression of GLUT4 in plasma membrane by flowcytometry (b). Representative Western blot gel documents and summarized data showing the protein expression of pIRS-1ser307 (c,d) and p-AKT (e) in HK-2 cells. *P < 0.05 vs. Ctrl; # P < 0.05 vs. PA treated group (n = 3).
