Figure 1 | Scientific Reports

Figure 1

From: Worsened outcome in patients with pancreatic ductal carcinoma on long-term diabetes: association with E-cadherin1 (CDH1) promoter methylation

Figure 1

Analysis by methylation-specific PCR in PDC. The subjects bearing promoter methylation of CDKN2A and CDH1 showed both methylated and unmethylated bands (A). Non-methylated tumor exhibited only unmethylated band. U = unmethylated, M = methylated (See also full-length gels for each gene in Supplementary Figure S2). Sequencing of the amplified PCR-products indicated methylated base of cytosine as C (black arrows), whereas C in unmethylated DNA was converted to thymidine (T) after bisulfide treatment (red arrows) (B). Lines under nucleotide sequence indicate primer sequence.

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