Figure 3
From: Domain swapping and SMYD1 interactions with the PWWP domain of human hepatoma-derived growth factor
Interactions between the PWWP domain and SMYD1. (A) Stereo view of DNA binding to symmetry-related molecules (upper panel). The PWWP-SMYD1 complex comprises chain A (green) and chain B (marine) and SMYD1 (orange). The symmetric related chain A (limon), chain B (blue) and DNA (grey) are generated. The detailed interactions between DNA and residues are shown in the enlarged panel. Gly22 and Lys80 from both C/N and N/C termini interact with DNA with the main-chain N atoms; other residues contact to DNA through their side chains. Arg79 binds to DNA with two hydrogen bonds, whereas the others utilize one. The involved residues and base pairs are shown as sticks with interactions in dashed lines. (B) Stereo view of the surface-charge distribution of the PWWP-SMYD1 complex. The positively charged region covers dimension of ~60 Å as measured from Arg79 to Gly22 with the main-chain structures, of which only the partial area at the end is utilized for DNA interactions.
