Figure 5
From: Domain swapping and SMYD1 interactions with the PWWP domain of human hepatoma-derived growth factor
The NCL-binding capabilities of HDGF and the apo PWWP domain. (A) The solid-phase binding assay of the apo HDGF, the HDGF-SMYD1 complex, the apo PWWP domain and the PWWP-SMYD1 complex. The designed diagram is shown in the upper panel, whereas the solid-phase binding result is shown in the lower panel. The NCL-binding capability of both HDGF and the PWWP domain is attenuated by SMYD1. (B) The designed diagram showing the competition of the PWWP domain and HDGF-Fc with NCL is shown in the upper panel. The PWWP domain exhibits competition ability for the interaction between HDGF-Fc and NCL; however, the competition capability is reduced by SMYD1, suggesting that the PWWP domain is essential for the HDGF-NCL interaction (lower panel). Values that differ significantly from controls are indicated as **P < 0.01 by one-way ANOVA.
