Figure 2 | Scientific Reports

Figure 2

From: Evidence for a radiation-responsive ‘p53 gateway’ contributing significantly to the radioresistance of lepidopteran insect cells

Figure 2

Sfp53 is incompetent of regulating cell cycle perturbations and expression of its transcriptional targets following irradiation. (a,b) Real time PCR on some of the canonical transcriptional targets of p53 showed its transcriptional inactivity after irradiation at early (4 h) and late (24 h) time points. The fold change values for MnSOD ranged from 0.8 to 1.45 (p = 0.103) and p21 fold change values ranged 0.9 to 1.4 (p = 0.076). (c) Cell cycle distribution using flow cytometry was performed on harvested cells 24 h post irradiation with or without siRNA against p53. No change in cell cycle distribution was observed with p53 siRNA indicating no participation of p53 in the regulation of cell cycle perturbation. Data are mean ± SD of three independent experiments.

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