Figure 4

Ectopic overexpression of Sfp53 induces caspase-3 dependent apoptosis in miR-31 independent manner. (a) Sf9 cells were harvested 24 h following transfection with Sfp53 clone. Significant cell death was observed under DIC microscope. Cells were then harvested and immuno-blotted with p53 specific antibody. Cell death/apoptosis was also measured 24 h post transfection by (b) caspase-3 activation and (c) morphological analysis. Data are mean ± SD of three independent experiments. (*P < 0.001, **P < 0.02) (d) Sf-miR-31 showed no change in expression, measured by q-PCR 24 h after Sfp53 clone transfection.