Figure 8
From: Characterization of Dopaminergic System in the Striatum of Young Adult Park2−/− Knockout Rats
The effect of parkin protein loss on the levels of transactivation DNA-binding protein (TDP-43). (A) The anti-TDP-43 antibody was validated in PC12 cells and found, as expected, in the nucleus. (B) The immunoreactivity of TDP-43 was detected as 43 kDa band and two other bands, ~52 kDa and ~70 kDa band. A weak, potentially non-specific band of ~26 KDa was also observed on the membranes. (C) The 43 kDa and 52 kDa bands did not differ in optical density between the PKO and WT rats while 70 kDa band showed a trend for lower density in the PKO rats compared to the controls (−37%, p < 0.1, two-way ANOVA with Holm-Sidak’s post hoc test, n = 6–9). The data are expressed as mean ± SEM).
