Figure 5 | Scientific Reports

Figure 5

From: Loss of the Mitochondrial Fatty Acid β-Oxidation Protein Medium-Chain Acyl-Coenzyme A Dehydrogenase Disrupts Oxidative Phosphorylation Protein Complex Stability and Function

Figure 5

The amount of newly-translated mtDNA-encoded subunits incorporated into the OXPHOS supercomplex is reduced in MCAD knockout mitochondria. MtDNA-encoded proteins were radiolabeled in both 143B control (CON) and 143B MCAD knockout (KO) cells in the presence of cycloheximide and chased for 0, 3 or 24 h. Mitochondria were then isolated for two dimensional (2D) native-PAGE analysis. OXPHOS complex subunits are identified according to14,38. Following 24 h chase, the incorporation of subunits ND1, ND2, ND3, ND4, ND5 and ND6 (complex I), Cyt b (complex III) and COI, COII and COIII (complex IV) into the CI/CIII2/CIV supercomplex is reduced in MCAD KO mitochondria compared to the control (CON) (p = 0.001).

Back to article page