Table 1 Optical microscopy (OM) slides examined to detect Trypanosoma cruzi infection in 841 triatomine bugs, central Brazil, 2012–2014. Fresh and Giemsa-stained slides were examined by entomological-parasitological routine surveillance (EPRS) and University of Brasília (UnB) staff in different combinations.

From: Surveillance of vector-borne pathogens under imperfect detection: lessons from Chagas disease risk (mis)measurement

OM slide combinations

Fresh slides

Stained slides

Bugs1

EPRS

UnB

EPRS

UnB

Fresh EPRS + Fresh UnB

  

19

Fresh EPRS + Stained EPRS

 

 

29

Fresh EPRS + Stained UnB

  

67

Fresh UnB + Stained UnB

 

 

22

Fresh EPRS + Fresh UnB + Stained UnB

 

334

Fresh EPRS + Stained EPRS + Stained UnB

 

370

Total (slides/bugs)

819

375

399

793

(2386/841)

  1. 1All tested also by one conventional and two real-time quantitative PCRs. •Slide read.