Table 2 Numbers of triatomine bugs tested and scored as positive for Trypanosoma cruzi infection by optical microscopy and molecular methods.

From: Surveillance of vector-borne pathogens under imperfect detection: lessons from Chagas disease risk (mis)measurement

Test

Triatomine bug species

P. megistus

T. sordida

R. neglectus

T. pseudomaculata

P. geniculatus

n

+

n

+

n

+

n

+

n

+

Fresh slide

EPRS

405

38

273

55

92

24

33

0

16

1

UnB

332

41

0

−

1

0

40

0

2

0

Stained slide

EPRS

21

3

273

60

91

25

0

−

14

0

UnB

395

41(12)

248

28(26)

89

16(16)

45

0(0)

16

0(0)

Microscopy1

EPRS

405

38

273

60

92

25

33

0

16

1

UnB

414

42

248

28

89

16

45

0

16

0

Total

415

64

273

60

92

25

45

0

16

1

cPCR

 

415

84

273

63

92

22

45

24

16

2

qPCR

1st

415

184

273

89

92

41

45

30

16

3

2nd

415

186

273

88

92

41

45

30

16

3

Molecular1

Total

415

186

273

89

92

41

45

30

16

3

Total1

 

415

215

273

100

92

48

45

30

16

4

  1. P., Panstrongylus; T., Triatoma; R., Rhodnius. EPRS, entomological-parasitological routine surveillance systems (state of Goiás and Federal District, Brazil); UnB, University of Brasília; cPCR, conventional PCR on the 24Sα subunit of the nuclear ribosomal DNA; qPCR, real-time PCR on nuclear satellite DNA For each species, the numbers of bugs tested (n) and scored as positive (+) are given; for Giemsa-stained microscope slides read at UnB, the number of bugs in which infection was unambiguously determined is also given (in parentheses). 1Bugs scored as positive in at least one test.
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