Figure 1

Generation of LCMV GP33 epitope expressing CT2A glioma line and Cl13-induced PD-1^hiGP33⁺CD8⁺ T cells. (A) Phenotypic analysis of GP33-specific CD8⁺ T cells in PBMCs of B6 mice infected with LCMV Arm or Cl13, 20 days after infection and before tumor challenge (see Fig. 2 for schematic). The plots are gated on Live⁺, CD3⁺ and CD8⁺ cells. Molecule on Y-axis (right subplots) denotes CD62L or PD-1(labeled top of subplots). Similar results were obtained from mice used in Fig. 2A. (B) Histograms showing the frequency of PD-L1, MHC Class I (H-2D^b) and II (I-A^b) molecules expression on clonal CT2A-gp33 cell populations after exposure to IFN-α, -β (each 500 units/mL) and -γ (20 µg/mL) (C) FACS plots showing the frequencies of intracellular IFN-γ expressing CD8⁺ lymphocytes, which were isolated from the spleen of LCMV Arm infected mice after 5 hours of no co-culture (left) or co-culture with CT2A (center) or CT2A-gp33 (right).