Figure 6

Aβ42 peptide-induced reduction in TSP-1 receptors was recovered by the addition of TSP-1 to hippocampal neurons. (A) After treatment of 2 μM Aβ42 peptide, hippocampal neurons were extracted and analysed by immunoblotting for NLGN1, α2δ-1, βIII-tubulin, NeuN, and SOD2 antibodies. β-Actin was used as a loading control. (B) Hippocampal neurons were treated with recombinant human TSP-1 (10, 50, 100, 250 ng/mL) under Aβ42 peptide treatment. mRNA expression levels of NLGN1 or α2δ-1 were analysed with RT-PCR. (mean ± SEM, **p < 0.005, n = 3 per group). (C) hUCB-MSC-administered mouse brains were extracted and analysed by immunoblotting with NLGN1 and α2δ-1 antibodies. β-Actin was used as a loading control. (WT: C57BL/6 J, CTRL: MEMα-administrated 5XFAD, MSC: hUCB-MSC-administered 5XFAD, n = 5 per group, *p < 0.05, **p < 0.005).