Figure 4

Transcriptome analysis of unstimulated whole blood after M. bovis AF2122/97 or M. tuberculosis H37Rv infection. (A) The number of differentially expressed genes in unstimulated whole blood for M. bovis- (blue) and M. tuberculosis- (purple) infected animals at day 42 -v- day 14 post infection (−1 > log₂FC < 1, FDR < 0.05). The green Venn diagram depicts the overlap of differentially expressed genes from the direct comparison of unstimulated whole blood samples from M. bovis and M. tuberculosis infected animals at day 14 and day 42 post infection (−1 > log₂FC < 1, FDR < 0.05). (B) The top 10 differentially expressed genes between M. bovis- (blue) and M. tuberculosis- (purple) infected animals at day 14- and day 42-post infection (FDR < 0.05). The graph depicts positive relative log₂ fold change values where a gene that shows increased expression in M. bovis infected animals is relative to its expression in M. tuberculosis infected animals and vice versa. (C) Pathway enrichment analysis results for the list of 523 differentially expressed genes between M. bovis and M. tuberculosis infected animals at day 14-post infection. The graph depicts the enrichment of each pathway in the differentially expressed gene list based on the SIGORA successes metric (circle size) and the number of genes annotated within the pathway (“#genes in pathway”) while the colour bar depicts the significance of the association (Bonferroni < 0.05). (D) The relative expression (transcripts per million, TPM, “log(TPM +1)”) and the relative change in expression (log₂ fold change (“Log₂FC”)) of the genes belonging to the Antigen activation of B cell Receptor (R-HSA-983695), Collagen degradation (R-HSA-1442490) and Extracellular matrix (“ECM”) organization (R-HSA-1474244) pathways enriched for the comparison of M. bovis and M. tuberculosis infected animals at day 14 post infection. Genes that pass multiple hypothesis testing are denoted with an asterisk (Benjamini-Hochberg, FDR < 0.05) (*).