Figure 3 | Scientific Reports

Figure 3

From: Functional Isolation of Tumor-Initiating Cells using Microfluidic-Based Migration Identifies Phosphatidylserine Decarboxylase as a Key Regulator

Figure 3

Whole transcriptome sequencing reveals genes differentially expressed between MDA-MB-231 migratory and non-migratory cells. (a) Gene expression of migratory and non-migratory MDA-MB-231 cells determined by next generation sequencing. Each dot represents a gene. Genes in the upper left and lower right corners correlate negatively and positively with migratory cells, respectively. (b) 5% serum chemo-attraction migration assay of ALDHbr, CD24−/low/CD44+ and non-TIC of MDA-MB-231 cells. Both ALDHbr and CD24−/low/CD44+ cells have higher motility than non-TICs. (n = 1,200 channels). ***refers to P < 0.001. (c,d) Comparison of significantly up-regulated and down-regulated genes of SUM159 and MDA-MB-231 cells. (e) The Kaplan-Meier plot shows that low levels of PISD expression correlate with reduced relapse-free survival (RFS) in breast cancer.

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