Figure 4
From: COX-2 induces oncogenic micro RNA miR655 in human breast cancer
Effects of miR655 knock-down and NOCTH/WNT inhibitors on SLC phenotypes of miR655 overexpressing cells: Spheroid formation in MCF7-COX-2-655KD and SKBR3-COX-2-655KD cells (achieved with transient miR655 knock-down), compared to respective Mock controls. (A) Morphology, (B) spheroid forming efficiency and spheroid sizes (perimeter) (C) of MCF7-COX-2 and SKBR3-COX-2 cells after miR655 knock-down. (D) Expression of miR655 in MCF7-miR655 cells after treating with inhibitors of NOTCH (DAPT) and WNT (rhDkk). (E) Representative images for MCF7-miR655 cells after above treatments demonstrating relative spheroid number (F) and perimeter (G). Quantitative data are presented as a mean of triplicate independent experiments ± SEM. *Indicates p < 0.05, **p < 0.005, ***p < 0.001 compared to parental and mock cell lines. Scale bar is 60 µm.
