Figure 2
Validation of internal quality control of the ddEGFR test. (A) Internal quality control (iQC) validation using reference standard. Each FFPE reference standard DNA extract for the EGFR mutations was blended with a fixed amount of wild-type gDNA (3.3 ng, 1,000GE) targeting a 1.5% mutation level, which was validated for use in the ddEGFR test. Values are expressed as the mean ± SD of nine experiments. (B) Internal quality control (iQC) validation using the reference standard. Four serial dilutions of each FFPE reference standard DNA extract were prepared and subjected to the ddEGFR test. Error bars indicate SD. Values are expressed as the mean ± SD of three experiments.
