Figure 2

Binding of CAN1/2/B Antibodies to various type/subtypes NA proteins. Allantoic fluids containing viruses were fractionated in SDS-PAGE. Four gels were run and transferred to 4 membranes in parallel, followed by detection of the NA proteins using the CAN1 antibodies (panel A), CAN2 antibodies (panel B), CANB antibodies (panel C). Rabbit polyclonal anti-NP (nucleoprotein) proteins of influenza A viruses were used as control (panel D). Note: Anti-NP antibody against type A cannot bind to the NP of type B viruses. Original pictures see Supplementary Figure S3.