Figure 3 | Scientific Reports

Figure 3

From: Universal type/subtype-specific antibodies for quantitative analyses of neuraminidase in trivalent influenza vaccines

Figure 3

Binding of subtype-specific antibodies to their respective reference antigens derived from multiple viral strains. The reference antigens used are described in Table S1. Lane 1–4: 4 strains of H1N1; Lane 5–8: 4 strains of H3N2; Lane 9–12: 4 strains of type B vaccines. The samples were fractionated on SDS-PAGE (1 μg HA/lane). Four gels were run and transferred to 4 membranes in parallel, followed by detection of the NA proteins in W.B. using the CAN1 (panel A) and CAN2 antibodies (panel B) and CANB antibodies (panel C). Rabbit polyclonal anti-NP (nucleoprotein) proteins of influenza A viruses were used as control (panel D). Note: Anti-NP antibody against type A cannot bind to the NP of type B viruses.

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