Table 2 ZIKV-positive pools for the different mosquito species. CPE appearance times and Ct values by RT-qPCR.

From: Zika Virus in Salivary Glands of Five Different Species of Wild-Caught Mosquitoes from Mexico

Mosquitoes genera and species

Number of mosquitoes

Pools presenting CPE

CPE (dpi)

Ct mean values in RT-qPCR for ZIKV (SD)

Aedes aegypti

25

Male

2

10.50 (0.06)

Ae. aegypti

8

SG

3

14.14 (1.40)

MG

3

13.87 (0.67)

B

3

13.39 (0.35)

Ae. aegypti

3

SG

3

12.97 (1.07)

MG

3

14.19 (0.95)

B

3

14.26 (0.47)

Ae. vexans

1

SG

3

11.56 (0.91)

MG

3

12.17 (0.56)

B

3

13.74 (0.09)

Culex quinquefasciatus

25

Male

2

11.91 (1.18)

Cx. quinquefasciatus

16

SG

3

14.36 (0.33)

MG

3

14.32 (0.35)

B

3

14.01 (0.18)

Cx. quinquefasciatus

20

SG

3

12.76 (0.43)

MG

5

12.16 (0.51)

B

3

14.49 (0.14)

Cx. quinquefasciatus

25

SG

3

13.23 (0.55)

MG

3

12.76 (0.22)

B

3

12.59 (0.24)

Cx. quinquefasciatus

9

SG

1

12.91 (1.79)

MG

1

12.74 (0.22)

B

2

13.74 (0.79)

Cx. quinquefasciatus

3

SG

1

10.54 (0.71)

MG

3

11.80 (1.19)

B

2

14.74 (0.18)

Cx. coronator

2

SG

3

12.65 (2.69)

MG

3

13.26 (0.40)

B

3

13.56 (0.09)

Cx. tarsalis

3

SG

4

12.16 (0.58)

MG

4

11.86 (0.58)

B

1

13.89 (0.18)

  1. All RT-qPCR reactions were performed in triplicate. Ct mean and standard deviation were calculated. SG, salivary gland; MG, midgut; B, body; CPE, cytopathic effect; dpi, days post-inoculation; SD, standard deviation.