Figure 2 | Scientific Reports

Figure 2

From: The bacterial Type III toxin-antitoxin system, ToxIN, is a dynamic protein-RNA complex with stability-dependent antiviral abortive infection activity

Figure 2

(A) Effect of ToxI-S on ToxN cleavage of ToxI-F. Purified ToxN was either used directly, or was incubated with ToxI-S in a 1:4 molar ratio for 15 minutes, prior to addition of the ToxI-F substrate. Samples taken at the indicated time points were quenched in RNA loading buffer and immediately flash-frozen in liquid N2. ToxI-F was cleaved into a ladder of bands and the rate appeared the same in both reactions. (B) Control reactions with an ompA RNA substrate, using the same ToxN and ToxI-S preparations as in A, showed the expected inhibition.

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