Figure 3 | Scientific Reports

Figure 3

From: The bacterial Type III toxin-antitoxin system, ToxIN, is a dynamic protein-RNA complex with stability-dependent antiviral abortive infection activity

Figure 3

ToxI is partially structured in solution. (A) 1D 1H spectra collected with ToxI in either native or denaturing (50% DMSO) buffer. The chemical shifts corresponding to base-paired imino protons are in the region of 10–14ppm. (B) 2D [1H, 15N]-HSQC spectrum of 15N-ToxI with predicted base assignments. (C) Schematic of internal bonding within the ToxI crystal structure, with key structural features, and interactions observed by NMR, indicated. The ToxI pseudoknot schematic shows the path of the RNA backbone in bold arrows, hydrophobic stacking interactions as dashed lines, Watson-Crick base pairs as two (A:U) or three (G:C) horizontal bars, and additional hydrogen bonds as grey lines, and is adapted from Blower et al.8.

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