Table 2 Bacterial strains, bacteriophages and plasmids used in this study.

Escherichia coli DH5α

K-12 strain: F⁻ Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_K⁻ m_K⁺) phoA supE44 λ⁻ thi-1 relA1 gyrA96

Invitrogen

Pectobacterium atrosepticum SCRI1043

Wild-type environmental isolate

³³

Serratia marcescens ATCC39006

Wild-type environmental isolate, carbapenem+, prodigiosin+

³⁴

ΦCDA [1–29]

Uncharacterised environmental P. atrosepticum phage, sewage isolate

This study

ΦCHI14[a-x]

Escape mutants of S. marcescens 39006 phage ΦCHI14

²³

pFLS6

toxIN-Y115A-FLAG in pBR322, Ap^R

⁸

pFLS90

toxIN locus in pBS KSII+, Ap^R

This study

pMJ4

toxIN-FLAG in pBR322, Ap^R

¹⁵

pTA46

toxIN in pBR322, Ap^R

⁷

pTA47

toxIN-FS in pBR322, Ap^R

⁷

pTA110

toxI in pBS KSII+ for antisense transcription, Ap^R

⁹

pTRB57

toxIN-FS-FLAG in pBR322, Ap^R

¹⁵