Figure 5 | Scientific Reports

Figure 5

From: Specific Phospholipids Regulate the Acquisition of Neuronal and Astroglial Identities in Post-Mitotic Cells

Figure 5

Phospholipids modulate the acquisition of neuronal and astroglial fates. Neurosphere derived-cells were incubated under differentiation condition plus PtdCho or PtdEtn for 3 days. (a) Representative images of cells stained with MAP2 (red), glial fibrillary acid protein (GFAP) (white), Nestin (green) and nuclei (DAPI) and visualized by confocal microscopy. The full pictures are included in a Supplementary Information file. (b) Percentage of neuronal-specified post-mitotic cells (Nestin positive/MAP2 positive/GFAP negative cells) after 3 days in culture. (c) Percentage of astrocyte-specified post-mitotic cells (Nestin positive/GFAP positive/MAP2 negative cells) after 3 days in culture. (d) Percentage of unspecified post-mitotic cells (Nestin positive/MAP2 negative/GFAP negative cells) after 3 days in culture. Data were presented as mean ± SEM *p < 0.05; **p < 0.01.

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