Figure 7
Neuronal differentiation is affected by blocking the PKA pathway. (a) Cells were cultured in media containing PtdCho in the presence or absence of the PKA inhibitor (KT5720 (10 μM)) and analyzed by immunofluorescence. Graph represents the percentage of neuronal differentiation measured in three independent experiments. (b) Cells were cultured in media containing PtdCho in the presence or absence of the PKA inhibitor (H89 (10 μM)). Graph represents the percentage of neuronal differentiation measured in two independent experiments. Data were presented as mean ± SEM *p < 0.05, **p < 0.01. (c) Western blot analysis was performed for p-CREB and γ-tubulin as a control. The gels/blots displayed here are cropped, and without high-contrast (overexposure). The full-length gels and blots are included in a Supplementary Information file.
