Table 1 Names and their primer sequences of markers used in the marker assisted selection of Zhengmai 7698 (the label √ means the existence of that gene).

From: Molecular marker assisted breeding and genome composition analysis of Zhengmai 7698, an elite winter wheat cultivar

Traits		Gene	Zhengmai	Parents			Markers and their primers			Reference
Superior traits	High quality gene	Gene	7698	4B269	Zhengmai 9405	Zhoumai 16	Marker	Forward primers (5′-3′)	Reverse primers (5′-3′)	Reference
High molecular weight glutenin subunits		*Ax1*	√	√			UMN19	CGAGACAATATGAGCAGCAAG	CTGCCATGGAGAAGTTGGA	¹⁰
		Ax-null			√	√	UMN19	CGAGACAATATGAGCAGCAAG	CTGCCATGGAGAAGTTGGA	¹⁰
	*Bx7*	*Bx7*	√	√	√	√	Bx7	CACTGAGATGGCTAAGCGCC	GCCTTGGACGGCACCACAGG	¹¹
	*By8*	*By8*		√	√		ZSBy8	TTAGCGCTAAGTGCCGTCT	TTGTCCTATTTGCTGCCCTT	¹²
		By9	√			√	ZSBy9a	TTCTCTGCATCAGTCAGGA	AGAGAAGCTGTGTAATGCC	¹²
		Dx2				√	UMN25	GGGACAATACGAGCAGCAAA	CTTGTTCCGGTTGTTGCCA	¹⁰
		*Dx5*	√	√	√		Dx5	CGTCCCTATAAAAGCCTAGC	AGTATGAAACCTGCTGCGGAC	¹³
		*Dy10*	√	√	√		UMN26	CGCAAGACAATATGAGCAAACT	TTGCCTTTGTCCTGTGTGC	¹⁰
		Dy12				√	UMN26	CGCAAGACAATATGAGCAAACT	TTGCCTTTGTCCTGTGTGC	¹⁰
Grain hardness		Pinb-D1a	√	√	√		Pinb-D1a	ATGAAGACCTTATTCCTCCTA	CTCATGCTCACAGCCGCC	¹⁴
Grain hardness		Pinb-D1b				√	Pinb-D1b	ATGAAGACCTTATTCCTCCTA	CTCATGCTCACAGCCGCT	¹⁴
Lipoxygenase		TaLox-B1a	√		√		LOX16	CCATGACCTGATCCTTCCCTT	GCGCGGATAGGGGTGGT	¹⁵
Lipoxygenase		TaLox-B1b		√		√	LOX18	ACGATGTGAGTTGTGACTTGTGA	GCGCGGATAGGGGTGC	¹⁵
Yellow pigment content		Psy-A1a				√	YP7A	GGACCTTGCTGATGACCGAG	TGACGGTCTGAAGTGAGAATGA	¹⁶
	*Psy-A1b*	*Psy-A1b*	√	√	√		YP7A	GGACCTTGCTGATGACCGAG	TGACGGTCTGAAGTGAGAATGA	¹⁶
		Psy-B1a	√		√	√	YP7B-1	GCCACAACTTGAATGTGAAAC	ACTTCTTCCATTTGAACCCC	¹⁷
	*Psy-B1b*	*Psy-B1b*		√			YP7B-1	GCCACAACTTGAATGTGAAAC	ACTTCTTCCATTTGAACCCC	¹⁷
		Psy1-D1g		√	√		YP7D-1	TCCGACACCATCACCAAGTTCC	CGTTGTAGGTTTGTGGGAGT	¹⁸
Polyphenol oxidase activity		PPO-A1a		√		√	PPO18	AACTGCTGGCTCTTCTTCCCA	AAGAAGTTGCCCATGTCCGC	¹⁹
	*PPO-A1b*	*PPO-A1b*	√		√		PPO18	AACTGCTGGCTCTTCTTCCCA	AAGAAGTTGCCCATGTCCGC	¹⁹
	*PPO-D1a*	*PPO-D1a*	√			√	PPO16	TGCTGACCGACCTTGACTCC	CTCGTCACCGTCACCCGTAT	²⁰
		PPO-D1b		√	√		PPO29	TGAAGCTGCCGGTCATCTAC	AAGTTGCCCATGTCCTCGCC	²⁰
Powdery mildew resistant		Pm2	√	√	√	√	Pm2	AGCTGTTTGGGTACAAGGTG	GCCATCGTTTTCTACTAG	²¹
		Pm4b	√		√	√	Pm4b	GTGGTGTATCAAATGTCATCAGTACTAC	TCCAGTGACCCCATCTGCTCATAC	²¹
		Pm8	√	√	√	√	Pm8	GGAGACATCATGAAACATTTG	CTGTTGTTGGGCAGAAAG	²¹
Yellow rust resistant		Yr9	√	√		√	Xgwm582	AAGCACTACGAAAATATGAC	TCTTAAGGGGTGTTATCATA	²²
Yellow rust resistant		YrZH84	√		√		Xcfa2040	TCAAATGATTTCAGGTAACCACT	TTCCTGATCCCACCAAACAT	²³
Pre-harvest sprouting		PHS1	√	√		√	PHS1	GGTGGAACAGATGCAACTAAAGG/ GGTGGAACAGATGCAACTAAAGA	GTGAGTGTTATATGAAACTAATGATCCATT	²⁴
resistant		PHS-4AL	√	√			PHS-4AL	TGGAGTCTGAAAGCATTCGA/TGGAGTCTGAAAGCATTCGG	TCCATGCATCATAGGAAAACA	²⁵

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Table 1 Names and their primer sequences of markers used in the marker assisted selection of Zhengmai 7698 (the label √ means the existence of that gene).

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