Figure 6
STS reduces CS-induced activation of ERK/NF-κB pathway in 16HBE cells and mouse lungs. (A) Levels of phosphorylated ERK1/2 in cytosol protein and levels of NF-κB in nuclear protein from lung tissue of mice exposed to CS with or without STS treatment. Full-length blots are presented in supplementary Figure 1A. *P < 0.05 and **P < 0.01, n = 3. CSE, cigarette smoke extract; CS, cigarette smoke. (B) 16HBE cells were treated with 2% CSE for 1 h with or without 2 h of STS pretreatment. Levels of ERK1/2 and phosphorylated ERK1/2 in cytosol protein and levels of NF-κB with histone H3 as loading control in nuclear protein. Full-length blots are presented in supplementary Figure 1B.
