Figure 9
Glucuronidase activity in cell lysates of recombinant S. lividans strains containing gusA under the control of the inducible P21-cmt promoter fused to different degradation tags. GUS wt – strain that contains wild type gusA gene; AAV - strain that contains GusA fused with AAV C-terminal tag; LVA – strain with GusA containing LVA C-terminal tag and ASV – strain with GusA fused to ASV C-terminal tag. The strains were grown in TSB medium for 2 days in the presence of the inducer, after which the biomass was collected, washed and grown in fresh TSB medium without the inducer for 2 days.
