Table 2 Primers used in this study.

From: A set of synthetic versatile genetic control elements for the efficient expression of genes in Actinobacteria

Primers

Sequence 5′-3′

Purpose

XbagusAForw

AAAATCTAGATACGCATATGCTGCGGCCCGTCGAAACC

Cloning the gusA gene without RBS and promoter

EVgusARev

AAAAGATATCTGCTTCCCGCCCTGCTGCGG

XbaRBSForw

AAAAGATATCGAAATCACTCCCAATTAATCTAG

Cloning randomly synthesized RBSs upstream of the gusA gene

NdeNRBSRev

AAAACATATGNNNNNNCCTCCNNNNNNNNNNTTTCTCATCCTAAAGAATCTCTC

NdeWRBSRev

AAAACATATGWWWWWWCCTCCWWWWWWWWWWTTTCTCATCCTAAAGAATCTCTC

NdeP72bezRBS

AAAACATATGTTTCTCATCCTAAAGAATCTCTC

Cloning the gusA gene with the P72 promoter but without RBS

RBSProg1Rev

AAAACATATGAATGAACCTCCTTCTTTCTTTTTCTCATCCTAAAGAATCTCTC

Cloning the gusA gene fused to in silico designed RBS

NdeRBS8Nrev

AAAACATATGTGTTTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC

Cloning gusA fused to synthetic RBSs

NdeRBS7Nrev

AAAACATATGTGTTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC

NdeRBS6Nrev

AAAACATATGTGTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC

NdeRBS5Nrev

AAAACATATGTGCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC

NdeRBS3NRev

AAAACATATGCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC

ApForw

TTTTTCTAGTTATATGAGTAAACTTGGTCT

Fusion the Apr gene with the P82 promoter

ApP82Rev

GCTACAATCCTACTTGAAGAATCCTAATTTTAGCCTCAGGAGACGAAAGGGCCTCGTGATA

EGFPNRBSRev

TTTTGATATCGAACAGCTCCTCGCCCTTGCTGGATCGGGATCCTTTTTCGAACTGCGGGTGGCTCCACATNNNNNNCCTCCNNNNNNNNNGCTACAATCCTACTTGAAGAATC

Cloning randomly synthesized RBSs upstream of the egfp gene

EGFPNRBS-9For

TTTTTCTAGATCCTGAGGCTAAAATTAGGATTCTTCAAGTAGGATTGTAGCTGACTAATGGGAGGCGTCTGATGTGGAGCCACCCGCAGTTC

EGFP fusion with NRBS-9

EGFPNRBS-131For

TTTTTCTAGATCCTGAGGCTAAAATTAGGATTCTTCAAGTAGGATTGTAGCATCGTAGGAGGAGGCAAAACATGTGGAGCCACCCGCAGTTC

EGFP fusion with NRBS-131

EGFPRev

GATATCTTACTTGTACAGCTCGTCCATGC

GusARevLVA

AAAAGATATCTTATCAGGCTACGAGGGCGAAGGCCTGCTGGGAGGAATCGCGC

gusA fusion with degradation tags

GusARevAAV

TTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGGAAAAGATATCTTATCAAACGGCAGCGGCGAAGGCCTGCTGGGAGGAATCGCGCTTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGG

GusARevASV

AAAAGATATCTTATCATACGGAAGCGGCGAAGGCCTGCTGGGAGGAATCGCGCTTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGG

GusASpeForw

AAAAACTAGTCGAGCAACGGAGGTAC

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